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International Journal of Infectious Diseases: Volume 2, Number 3
Outbreak of Salmonella enteritidis Gastroenteritis: Investigation by Pulsed- Field Gel Electrophoresis
Kwai-Lin Thong, PhD; Savithri Puthucheary, MBBS; and Tikki Pang, PhD

Int J Infect Dis 1998; 2(3):159-163.

Objective: Pulsed-field gel electrophoresis (PFGE) was used to investigate an outbreak of gastroenteritis caused by Salmonella enteritidis. The outbreak occurred among university undergraduates who consumed contaminated food. Method: Molecular typing was done by analyzing DNA band patterns of isolates of S. enteritidis after digestion of chromosomal DNA with infrequently-cutting restriction endonucleases XbaI, AvrII, and SpeI and separation of DNA fragments using PFGE. Results: Twenty-nine outbreak isolates of S. enteritidis had identical or highly similar PFGE patterns, whereas different PFGE patterns were observed among three epidemiologically unrelated isolates obtained during the same period. Conclusion: The data obtained confirm the value of PFGE in epidemiologic investigations of outbreaks caused by S. enteritidis.

KEY WORDS: gastroenteritis, PFGE typing, S. enteritidis

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