[an error occurred while processing this directive]
International Journal of Infectious Diseases: Volume 2, Number 3
Outbreak of Salmonella enteritidis Gastroenteritis: Investigation by Pulsed- Field Gel Electrophoresis
Kwai-Lin Thong, PhD; Savithri Puthucheary, MBBS; and Tikki Pang, PhD

Int J Infect Dis 1998; 2(3):159-163.

Objective: Pulsed-field gel electrophoresis (PFGE) was used to investigate an outbreak of gastroenteritis caused by Salmonella enteritidis. The outbreak occurred among university undergraduates who consumed contaminated food. Method: Molecular typing was done by analyzing DNA band patterns of isolates of S. enteritidis after digestion of chromosomal DNA with infrequently-cutting restriction endonucleases XbaI, AvrII, and SpeI and separation of DNA fragments using PFGE. Results: Twenty-nine outbreak isolates of S. enteritidis had identical or highly similar PFGE patterns, whereas different PFGE patterns were observed among three epidemiologically unrelated isolates obtained during the same period. Conclusion: The data obtained confirm the value of PFGE in epidemiologic investigations of outbreaks caused by S. enteritidis.

KEY WORDS: gastroenteritis, PFGE typing, S. enteritidis

Back to Table of Contents



The International Journal of Infectious Diseases is owned by
the International Society for Infectious Diseases.
The Journal is published quarterly for the ISID by B.C. Decker Inc.